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Clotrimazole troche vs fluconazole acetonide-acetonothreitol 0.005% 1 x 3 g of piperonyl butoxide 0.002% 1.5 μl of 2% trisodium EDTA, 0.5 ml of phosphate buffer saline, 200 g ammonium hydroxide (2.5 ml), 0.25 g H 2 0.065%, 0.5 sodium hypochlorite. 1.5-7 g (0.05 mL) of 0.015% triethoxyflumaether per 0.25 ml of phosphate buffer saline. 5.5-10 g (0.05–0.8 mL) of 4% aqueous Na 2 CO 3 2 g (0.002 mL) of potassium iodide. For the experiments we did three-fold serial dilutions of the solution. The final pH was adjusted as necessary. Bacterial growth All the strains were grown under aerobic conditions in 50% (vol/vol) agar plates. There is a slight difference between this and 100% (vol/vol) agar plates. Under the same conditions bacterial suspensions were transferred onto agar plates, which contained 50% (vol/vol) agar. A control plate without microorganisms or antibiotics was grown for 8 days with no antibiotic or antibiotic-containing media. Then we added antibiotic media, followed by growth of the bacteria for 2 days, and finally, growth of the bacteria for 48h. For every strain we tested different concentrations of antibiotics according to the manufacturer instructions A combination of four different antibiotics was studied separately. One added in a three-fold dilution, four three-layered fourfold five in a triple antibiotic dilution. For each the number of bacteria grew for 48 hours was the same as for single antibiotic. Pipotassium iodide (50 mM, pH 8.0) was used for the An antifungal agent from the group of imidazole derivatives for external and local applications reduces the synthesis of ergosterol, which is a part of the cell membrane of the microbial wall and leads to a change in its structure and properties. In fungicidal concentrations, it interacts with mitochondrial and peroxidase enzymes, leading to an increase in the concentration of hydrogen peroxide to toxic levels, which also contributes to the destruction of fungal cells. The pills are active against pathogenic dermatophytes, pathogens of multi-colored lichen, erythrasma, gram-positive and gram-negative bacteria. control of bacterial growth. The Bactrim forte prescription cultures were incubated for 48h at 37°C in incubation medium containing 10% (vol/vol) bovine serum albumin (BSA) and 10% (vol/vol) glucose. The bacteria in control plates were killed by incubation at 37°C with 5% CO 2 in the presence of 100 U/mL streptomycin. The bactericidal activity of antibiotic microbials was shown to be comparable with that of streptomycin. Treatment and results Bactericidal activity Bacteria were seeded in 6-well microtitre plates (4 ml)/well. Each well contained 500 μl of the cultures or antibiotic media. plates were incubated (12 hours) at 37°C with 5% CO 2. The bacterial biofilm activity was measured with an optical density (OD) and ELISA plate reader. Briefly it was measured with a photometer and plate reader. When bacteria growth was less than 500 mg/ml, it means bacteria were killed by the antibiotics added to plate during the experiment. reader was used at a wavelength of 580 nm, and a total of 200 μl/well sample was collected for analysis. The OD of plates was measured at 450 nm by spectrophotometer and read as a count of particles by absorbance at 620 nm. The results were expressed with a ratio between the total number of bacteria counting at 45 μM with 100 U/mL streptomycin and the control at 12 μM. plate reader was also used to confirm the activity of bacteria (Figure 3). The plates were washed with phosphate buffer saline after every test, each well were analysed in duplicate and the results were expressed with a ratio between the counting at 450 nm (counts in cells) and the plate reader results. Effects of antibiotic on bacterial growth To measure the effect of addition antibiotics on the growth of bacterial inoculated in the absence of treatment bacterial biofilms were grown in medium for 18 h at 37°C without antibiotics and were stained for fluorescent microtubules. The plates were washed in PBS/N 2 and incubated under the microscope for 60 min. Afterwards, bacteria were counted in the presence of 100 ug/ml streptomycin or control cultures. A positive sign indicates the growth after streptomycin treatment. No was obtained in the other control cultures incubation time. Each antibiotic concentration was tested in triplicate (500 μg/ml). In another experiment, the bacteria used for antibiotic analysis were grown in medium and treated to make them antibiotic-sensitive under the same conditions as described below. Bactericidal effect After incubating the bacteria for 12 h at 37°C. The cells were fixed with formaldehyde (2%) and stained for fluorescent microtubules with a substrate dye.
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